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xtractor trademark buffer kit  (TaKaRa)


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    Structured Review

    TaKaRa xtractor trademark buffer kit
    Xtractor Trademark Buffer Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/xtractor+buffer+kit/xTractor+Buffer+Kit/us12545935-514-17-21
    Average 96 stars, based on 24 article reviews
    xtractor trademark buffer kit - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Recombinant:

    Article Title: Different hypersensitivities against homologous proteins of MGL_1304 in patients with atopic dermatitis.
    Article Snippet: These DNA fragments were digested by Kpn I/Hind III or Kpn I/Xho I, respectively, and then ligated into the same sites of pCold TF vector (Takara Bio). .. The recombinant proteins of Mala s 8 and Mala r 8 were generated by E. coli (JM109, Takara Bio) as previously described.3 Briefly, the cultures from single clones of JM109 which contained pCold TF vectors were lysed with xTractor buffer kit (Takara Bio) and purified by HisTARONTM gravity columns purification kit (Takara Bio), according to the manufacturer's instructions. .. Protein concentrations were measured by PierceTM BCA protein assay kit (Thermo Fisher Scientific, Waltham, MA, USA).

    Article Title: Seasonal composition of immature germ cells in the Yesso scallop identified by vasa-like gene (my-vlg) and protein expression, with evidence of irregular germ cell differentiation accompanied with a high mortality event
    Article Snippet: Amplified fragments were then subcloned into the E. coli expression vector pCold Pros2 DNA (Takara Bio Inc.) using the In-Fusion HD cloning kit (Takara Bio Inc.) according to manufacturer’s instructions. .. The recombinant my-Vasa produced from a transformed BL21 strain (Takara Bio Inc.) was extracted with the xTractor buffer kit (Takara Bio Inc.) and purified with Ni-NTA agarose resin (Qiagen, Tokyo, Japan). .. Prior to immunization, the His-tag was digested with HRV 3C protease (Takara Bio Inc.) and affinity purified from the recombinant protein using Ni-NTA agarose resin (Qiagen) to prevent unwanted antibody production against Pros2 protein.

    Article Title: Type-I-hypersensitivity to 15 kDa, 28 kDa and 54 kDa proteins in vitellogenin specific to Gadus chalcogrammus roe.
    Article Snippet: The DNA fragments were double-digested with XhoI and PstI, then ligated into pCold TF DNA (Takara Bio). .. For bacterial expression of pCold TFDNA by using Escherichia (E.) coli, BL21 (Takara Bio), cultures from the clone of BL21 that contained constructed plasmid vectors were lysed with xTractor buffer kit (Takara Bio), and the polyhistidine tagged- and TF-fused recombinant Pv (rPv) and b0-c (rb0-c) were extracted by HisTARON gravity columns purification kit (Takara Bio), according to the manufacturer's instructions. ..

    Generated:

    Article Title: Different hypersensitivities against homologous proteins of MGL_1304 in patients with atopic dermatitis.
    Article Snippet: These DNA fragments were digested by Kpn I/Hind III or Kpn I/Xho I, respectively, and then ligated into the same sites of pCold TF vector (Takara Bio). .. The recombinant proteins of Mala s 8 and Mala r 8 were generated by E. coli (JM109, Takara Bio) as previously described.3 Briefly, the cultures from single clones of JM109 which contained pCold TF vectors were lysed with xTractor buffer kit (Takara Bio) and purified by HisTARONTM gravity columns purification kit (Takara Bio), according to the manufacturer's instructions. .. Protein concentrations were measured by PierceTM BCA protein assay kit (Thermo Fisher Scientific, Waltham, MA, USA).

    Clone Assay:

    Article Title: Different hypersensitivities against homologous proteins of MGL_1304 in patients with atopic dermatitis.
    Article Snippet: These DNA fragments were digested by Kpn I/Hind III or Kpn I/Xho I, respectively, and then ligated into the same sites of pCold TF vector (Takara Bio). .. The recombinant proteins of Mala s 8 and Mala r 8 were generated by E. coli (JM109, Takara Bio) as previously described.3 Briefly, the cultures from single clones of JM109 which contained pCold TF vectors were lysed with xTractor buffer kit (Takara Bio) and purified by HisTARONTM gravity columns purification kit (Takara Bio), according to the manufacturer's instructions. .. Protein concentrations were measured by PierceTM BCA protein assay kit (Thermo Fisher Scientific, Waltham, MA, USA).

    Purification:

    Article Title: Different hypersensitivities against homologous proteins of MGL_1304 in patients with atopic dermatitis.
    Article Snippet: These DNA fragments were digested by Kpn I/Hind III or Kpn I/Xho I, respectively, and then ligated into the same sites of pCold TF vector (Takara Bio). .. The recombinant proteins of Mala s 8 and Mala r 8 were generated by E. coli (JM109, Takara Bio) as previously described.3 Briefly, the cultures from single clones of JM109 which contained pCold TF vectors were lysed with xTractor buffer kit (Takara Bio) and purified by HisTARONTM gravity columns purification kit (Takara Bio), according to the manufacturer's instructions. .. Protein concentrations were measured by PierceTM BCA protein assay kit (Thermo Fisher Scientific, Waltham, MA, USA).

    Article Title: Seasonal composition of immature germ cells in the Yesso scallop identified by vasa-like gene (my-vlg) and protein expression, with evidence of irregular germ cell differentiation accompanied with a high mortality event
    Article Snippet: Amplified fragments were then subcloned into the E. coli expression vector pCold Pros2 DNA (Takara Bio Inc.) using the In-Fusion HD cloning kit (Takara Bio Inc.) according to manufacturer’s instructions. .. The recombinant my-Vasa produced from a transformed BL21 strain (Takara Bio Inc.) was extracted with the xTractor buffer kit (Takara Bio Inc.) and purified with Ni-NTA agarose resin (Qiagen, Tokyo, Japan). .. Prior to immunization, the His-tag was digested with HRV 3C protease (Takara Bio Inc.) and affinity purified from the recombinant protein using Ni-NTA agarose resin (Qiagen) to prevent unwanted antibody production against Pros2 protein.

    Article Title: Recombinant expression using the tetrathionate hydrolase promoter in Acidithiobacillus ferrooxidans.
    Article Snippet: 1389-1723/$ https://doi.or In the ironand sulfur-oxidizing acidophilic chemolithoautotrophic bacterium, Acidithiobacillus ferrooxidans, tetrathionate hydrolase gene (Af-tth) is highly expressed during tetrathionate growth.. The expression levels of Af-tth were specifically determined by quantitative reverse transcription-polymerase chain reaction and the expression ratios of S0/ Fe2D and S4O6/Fe were found to be 68 ± 21 and 181 ± 5, respectively.. The transcriptional start site was identified by primer extension.

    Article Title: Type-I-hypersensitivity to 15 kDa, 28 kDa and 54 kDa proteins in vitellogenin specific to Gadus chalcogrammus roe.
    Article Snippet: The DNA fragments were double-digested with XhoI and PstI, then ligated into pCold TF DNA (Takara Bio). .. For bacterial expression of pCold TFDNA by using Escherichia (E.) coli, BL21 (Takara Bio), cultures from the clone of BL21 that contained constructed plasmid vectors were lysed with xTractor buffer kit (Takara Bio), and the polyhistidine tagged- and TF-fused recombinant Pv (rPv) and b0-c (rb0-c) were extracted by HisTARON gravity columns purification kit (Takara Bio), according to the manufacturer's instructions. ..

    Produced:

    Article Title: Seasonal composition of immature germ cells in the Yesso scallop identified by vasa-like gene (my-vlg) and protein expression, with evidence of irregular germ cell differentiation accompanied with a high mortality event
    Article Snippet: Amplified fragments were then subcloned into the E. coli expression vector pCold Pros2 DNA (Takara Bio Inc.) using the In-Fusion HD cloning kit (Takara Bio Inc.) according to manufacturer’s instructions. .. The recombinant my-Vasa produced from a transformed BL21 strain (Takara Bio Inc.) was extracted with the xTractor buffer kit (Takara Bio Inc.) and purified with Ni-NTA agarose resin (Qiagen, Tokyo, Japan). .. Prior to immunization, the His-tag was digested with HRV 3C protease (Takara Bio Inc.) and affinity purified from the recombinant protein using Ni-NTA agarose resin (Qiagen) to prevent unwanted antibody production against Pros2 protein.

    Transformation Assay:

    Article Title: Seasonal composition of immature germ cells in the Yesso scallop identified by vasa-like gene (my-vlg) and protein expression, with evidence of irregular germ cell differentiation accompanied with a high mortality event
    Article Snippet: Amplified fragments were then subcloned into the E. coli expression vector pCold Pros2 DNA (Takara Bio Inc.) using the In-Fusion HD cloning kit (Takara Bio Inc.) according to manufacturer’s instructions. .. The recombinant my-Vasa produced from a transformed BL21 strain (Takara Bio Inc.) was extracted with the xTractor buffer kit (Takara Bio Inc.) and purified with Ni-NTA agarose resin (Qiagen, Tokyo, Japan). .. Prior to immunization, the His-tag was digested with HRV 3C protease (Takara Bio Inc.) and affinity purified from the recombinant protein using Ni-NTA agarose resin (Qiagen) to prevent unwanted antibody production against Pros2 protein.

    Lysis:

    Article Title: Recombinant expression using the tetrathionate hydrolase promoter in Acidithiobacillus ferrooxidans.
    Article Snippet: 1389-1723/$ https://doi.or In the ironand sulfur-oxidizing acidophilic chemolithoautotrophic bacterium, Acidithiobacillus ferrooxidans, tetrathionate hydrolase gene (Af-tth) is highly expressed during tetrathionate growth.. The expression levels of Af-tth were specifically determined by quantitative reverse transcription-polymerase chain reaction and the expression ratios of S0/ Fe2D and S4O6/Fe were found to be 68 ± 21 and 181 ± 5, respectively.. The transcriptional start site was identified by primer extension.

    Affinity Column:

    Article Title: Recombinant expression using the tetrathionate hydrolase promoter in Acidithiobacillus ferrooxidans.
    Article Snippet: 1389-1723/$ https://doi.or In the ironand sulfur-oxidizing acidophilic chemolithoautotrophic bacterium, Acidithiobacillus ferrooxidans, tetrathionate hydrolase gene (Af-tth) is highly expressed during tetrathionate growth.. The expression levels of Af-tth were specifically determined by quantitative reverse transcription-polymerase chain reaction and the expression ratios of S0/ Fe2D and S4O6/Fe were found to be 68 ± 21 and 181 ± 5, respectively.. The transcriptional start site was identified by primer extension.

    Chromatography:

    Article Title: Recombinant expression using the tetrathionate hydrolase promoter in Acidithiobacillus ferrooxidans.
    Article Snippet: 1389-1723/$ https://doi.or In the ironand sulfur-oxidizing acidophilic chemolithoautotrophic bacterium, Acidithiobacillus ferrooxidans, tetrathionate hydrolase gene (Af-tth) is highly expressed during tetrathionate growth.. The expression levels of Af-tth were specifically determined by quantitative reverse transcription-polymerase chain reaction and the expression ratios of S0/ Fe2D and S4O6/Fe were found to be 68 ± 21 and 181 ± 5, respectively.. The transcriptional start site was identified by primer extension.

    Expressing:

    Article Title: Type-I-hypersensitivity to 15 kDa, 28 kDa and 54 kDa proteins in vitellogenin specific to Gadus chalcogrammus roe.
    Article Snippet: The DNA fragments were double-digested with XhoI and PstI, then ligated into pCold TF DNA (Takara Bio). .. For bacterial expression of pCold TFDNA by using Escherichia (E.) coli, BL21 (Takara Bio), cultures from the clone of BL21 that contained constructed plasmid vectors were lysed with xTractor buffer kit (Takara Bio), and the polyhistidine tagged- and TF-fused recombinant Pv (rPv) and b0-c (rb0-c) were extracted by HisTARON gravity columns purification kit (Takara Bio), according to the manufacturer's instructions. ..

    Construct:

    Article Title: Type-I-hypersensitivity to 15 kDa, 28 kDa and 54 kDa proteins in vitellogenin specific to Gadus chalcogrammus roe.
    Article Snippet: The DNA fragments were double-digested with XhoI and PstI, then ligated into pCold TF DNA (Takara Bio). .. For bacterial expression of pCold TFDNA by using Escherichia (E.) coli, BL21 (Takara Bio), cultures from the clone of BL21 that contained constructed plasmid vectors were lysed with xTractor buffer kit (Takara Bio), and the polyhistidine tagged- and TF-fused recombinant Pv (rPv) and b0-c (rb0-c) were extracted by HisTARON gravity columns purification kit (Takara Bio), according to the manufacturer's instructions. ..

    Plasmid Preparation:

    Article Title: Type-I-hypersensitivity to 15 kDa, 28 kDa and 54 kDa proteins in vitellogenin specific to Gadus chalcogrammus roe.
    Article Snippet: The DNA fragments were double-digested with XhoI and PstI, then ligated into pCold TF DNA (Takara Bio). .. For bacterial expression of pCold TFDNA by using Escherichia (E.) coli, BL21 (Takara Bio), cultures from the clone of BL21 that contained constructed plasmid vectors were lysed with xTractor buffer kit (Takara Bio), and the polyhistidine tagged- and TF-fused recombinant Pv (rPv) and b0-c (rb0-c) were extracted by HisTARON gravity columns purification kit (Takara Bio), according to the manufacturer's instructions. ..



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